Immunohistochemistry

EPL offers high quality immunohistochemical staining services using standard protocols developed in-house or transferred from the client. As many studies submitted to EPL do not have a protocol available for the intended antigen, species, or tissue to be studied, we offer support in developing new protocols or optimizing existing immunohistochemistry protocols. This may involve serial dilution of the antibody, variations in antigen retrieval, and troubleshooting. Costs for protocol development are based on time and materials devoted by EPL to the project. The optimized protocol can then be used for subsequent staining of study samples.


We do not currently accept human tissues.

We use a DAKO® autostainer for IHC staining of paraffin-embedded and frozen tissue sections from a variety of species. This system allows us to automate staining methods and has the capability to process up to 48 slides at one time, allowing for consistent and dependable results. The system is highly versatile with the ability to vary multiple parameters within a single run.

Digital imaging, image analysis and semi-quantitative evaluation of IHC stained slides are available upon request. We are currently developing protocols for producing and evaluating immunofluorescent-stained sections.

Standard protocols have been developed for a number of antibodies and are listed below.

 

Actin
alpha-synuclein
ß-Catenin
BrdU
CAR
CD4
CD8 (OX-8)
Complement C3
COX-2
Cyclin D1
Cytokeratin (WSS)
Desmin
F4/80
Factor VIII
Gamma-H2AX
GFAP
Glucagon
HAM56
Hsp60
Hsp90
Iba 1
Insulin
JNK
Ki-67
LAMP-1
Luteinizing Hormone (LH)
MAC-2
MHC-Class II (ox-6)
Myoglobin
Neurofilament
OLIG2
p27 Kip1
PCNA
PKC Alpha
S-100
S-100 (Beta)
SMN
TUNEL
Vimentin

 

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